Soft tissue, embryonal rhabdomyosarcoma, H&E stain

Rhabdomyosarcoma is a malignant tumour of skeletal muscle differentiation of which embryonal is the most common type, comprising 60% of cases. The tumor is most common in children under the age of 10 and is most frequently seen in the head and neck region or genitourinary tract. A wide range of differentiation may be seen. Many tumors have a primitive ‘small round blue cell’ appearance, with alternating areas of myxoid hypocellularity, while others have a predominant spindle cell morphology (seen here) with more easily identifiable rhabdomyoblasts.

Sections demonstrate a malignant, large spindle cell neoplasm centred on the spermatic cord with fascicular
architecture and moderate-to-marked pleomorphism (anaplasia) and occasionally multiple nucleoli. These features signify the anaplastic variant in this case, which is associated with a worse prognosis. Scattered elongated rhabdomyoblasts with cytoplasmic cross-striations and globoid rhabdomyoblasts are identified. In many cells the cytoplasm appears vacuolated. Mitotic figures are easily identified (13/10 HPF), including atypical figures. Necrosis is focally present.

By immunohistochemistry the tumor cells are positive for SMA, desmin, and myogenin (MYF4), confirming skeletal muscle differentiation. Molecular testing failed to detect the two most common translocations associated with alveolar rhabdomyosarcoma (PAX3-FOXO1; PAX7-FOXO1), essentially excluding this subtype from the differential diagnosis.

This slide shows H&E stain. See Related Content for SMA, desmin, and myogenin stains.