Nasal Sinus, Invasive aspergillosis, GMS stain

Nasal Sinus, Invasive aspergillosis, H&E stain
Details
Disease Category
Gender
Organ System/Discipline
Clinical History

75 year-old woman with history of sinonasal mass.

Case Discussion

Invasive aspergillosis is an aggressive fungal infection that results in tissue necrosis and destruction of the sinonasal tract. It most commonly presents in patients whose immune system has been compromised. Early diagnosis and treatment includes debridement and IV antifungals. These are critical in decreasing the risk of morbidity and mortality.

Although fungi can be seen on routine tissue sections, speciation of fungi requires fungal culture. Aspergillus fungi have thin, septate hyphae (2-5 µm), with acute angle branching (45°) or dichotomous branching. In contrast, Mucor fungi have larger, non-septate hyphae (7-20 µm), with wider angle branching up to 45-90°.

Histochemical staining such as GMS and PAS are used to detect fungi. In-situ hybridization is also used in cases with negative cultures.

This slide shows H&E stain. See Related Content for GMS stain.

Diagnosis
Invasive aspergillosis
Nasal Sinus, Invasive aspergillosis, GMS stain
Details
Disease Category
Gender
Organ System/Discipline
Clinical History

75 year-old woman with history of sinonasal mass.

Case Discussion

Invasive aspergillosis is an aggressive fungal infection that results in tissue necrosis and destruction of the sinonasal tract. It most commonly presents in patients whose immune system has been compromised. Early diagnosis and treatment includes debridement and IV antifungals. These are critical in decreasing the risk of morbidity and mortality.

Although fungi can be seen on routine tissue sections, speciation of fungi requires fungal culture. Aspergillus fungi have thin, septate hyphae (2-5 µm), with acute angle branching (45°) or dichotomous branching. In contrast, Mucor fungi have larger, non-septate hyphae (7-20 µm), with wider angle branching up to 45-90°.

Histochemical staining such as GMS and PAS are used to detect fungi. In-situ hybridization is also used in cases with negative cultures.

This slide shows GMS stain. See Related Content for H&E stain.

Diagnosis
Invasive aspergillosis